With Wes you only need 3 L of sample per capillary, so you can maximize the data you get per experiment. Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. Peaks are automatically defined by name or pI. Featured in well over 1500 publications and rapidly growing, Simple Western is a proven technology in all types of research applications. $message = SendPassword($link, $user, $login); Is your current method unable to handle the throughput you need? Low Sample Volume. Scientist, Vividion Therapeutics, "I used Simple Western because the protein samples I had were small. Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Maximize your time and sample, and get the information you need in one shot, by multiplexing. ", Melyssa Bratton, Ph.D., ", Dr. Mario Rothbauer, Are your assays lacking the reproducibility and reliability that you need to be confident in your research? $title = 'Simple Western antibody database :: ProteinSimple'; ", Jon Voutila, Ph.D., Postdoctoral Research Scientist, Institute of Cancer Sciences, University of Glasgow, "I can run immunoassays on Wes for an entire animal experiment in a single day. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and NIH 3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. Generate up to 8 data points with just 5 L. Simple Western instrument software Compass for Simple Western analyzes Simple Western Size or Charge assay data and processes the results for you. Submit new antibody validation Simple WesternSimple Western , Simple Western (). What tissue homogenization buffer can be used for Simple Western. Adjust the lane order and displayed molecular weight, stack multiple channel views, merge lane labels and control where they are located, and add additional label groups to add treatment or time-course information. Bio-Techne uses cookies to provide you with a great website experience. ProteinSimple branded instruments are available to quote. ", Mary G. Johlfs, M.S., RePlex with Abby enables multiple targets to be probed within the same experiment, saving time and money while conserving precious sample. to probe scWest chips. Postdoctoral Researcher, CSU Fullerton, "[Wes] Provides quantitative data, saves time and uses far less sample. Simple Western: What is the difference? GoToPage("index.html?status=$status"); Additional Wes information and documents may be found below. What's even better is that [Simple Western] can run up to 40 proteins in three days. Check out other antibodies that have been screened and tested with Simple Western Charge- and Size-based assays! Let Abby move your Westerns into the 21st century! Custom Targeted Protein Degradation Services, Certificate of Analysis (COA) Finder Tool, Simple Western Reagents, Kits, and Modules, Stellar High Sensitivity NIR/IR Detection. Is yoursample too precious or too expensive? multiple antibodies have been validated for the same target, the best Listed sample types are not Utilizing the RePlex module even further increases this throughput as I often probe for 5-6 proteins per capillary in a single run including phosphorylated/total protein pairs in the same lane. Traditional Western blotting steps and incubations, like sample separation,blocking, wash buffer incubations, incubations of primary and secondary antibodies, and detection of target proteins are precisely controlled inside a hands-free capillary electrophoresis instrument. By continuing to use this website you acknowledge this and agree to our cookie policy. // Custom page code By continuing to use this website you acknowledge this and agree to our cookie policy. ", Yotis Senis, Ph.D., If you are interested in theSimple Western Wes instrument, please view the comparison of Simple WesternJessandAbbyinstruments below. Director of Research Operations/Scientist, Roseman University of Health Sciences, "Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. With Wes, we can do multiple assays with one sample collection. Novus Biologicals branded products are not currently available on biotechne.com and can be found at novusbio.com. Spend more time thinking about your next paper and less time interpreting the results. My antibody is validated for Western blot. $title = 'Simple Western antibody database :: ProteinSimple'; Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and cIAP1/HIAP2 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. View our complete portfolio of Simple Western validated antibodies. Milo is an open platform so you can use any Western-validated antibody The Simple WesternTM antibody database is a user-interactive listing of Chemiluminescent detection gives you picogram-level sensitivity, letting you maximize the data you get from your sample. Postdoctoral Research Scientist, Institute of Cancer Sciences, University of Glasgow, "I can run immunoassays on Wes for an entire animal experiment in a single day. It would take about a week to run 40 proteins using Western blotting. To learn about Simple Western Systems, Simple Western is your protein characterization problem-solver. ProteinSimple will continue service and support of the Wes platform through July 30th, 2026. Detection of Human cIAP 1/HIAP 2 by Simple Western. // The code continues at the bottom of the page CleanHTTPVars(); ", Dr. Matteo Ferrari, ", Irene Tobias, Ph.D., ", Joseph Klebba, Ph.D., Check out the Simple Western FeliX fromAnalytik Jena for one example on how Simple Western pairs with liquid handling automation. ", Dr. Mario Rothbauer, Research Fellow, Brain Research Institute Monash Sunway (BRIMS), "It saves so much time! data to the Simple Western antibody database and receive a free 8x25 This database is intended to provide general guidance in identifying and selecting antibodies to test. ", Jamie Boisvenue, In exchange for 30 minutes of easy experimental setup, you get the results of 24 fully-analyzed samples after 3 hours of hands-free run time. Data analysis with Compasss user-friendly software is quick and easy. If your antibody hasnt been validated for Simple Western, you can submit your results to our Innovator's Reward Program for a voucher for the full purchase price for Risk-Free Testing. Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna, "It can be difficult to optimize new antibodies, particularly for brain markers where you have a lot of background noise and low levels of your target. The Simple Western Antibody Database on Bio-Techne.com is a user-interactive listing of all Simple Western validated antibodies to date. exhaustive. SIMPLE WESTERN CERTIFIED ANTIBODY DATASHEET View Antibody Link This antibody is certified for Gel-Free, Blot Free, Hands Free Simple Western Systems. Proteins can also be displayed as an electropherogram in graph view and data for multiple samples can be overlaid. development guidance for identifying and selecting antibodies to test. With HDR detection profile and analysis Wes gets you up to 6 logs of dynamic range. $page = GetParamDefault("page", $defpage); Department of Immunology, Landspitali, the National University Hospital Iceland, "Our lab came across ProteinSimples Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables. Cardiovascular Research Technician, Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry, "The Simple Western Charge assay, a capillary isoelectric focusing immunoassay, exceeded the reliability of 2D Western blots for resolving recombinant PKG-I and PKG-I and uses 100,000X less sample quantity, making it well-suited for clinical disease proteomics because protein isoforms and post-translational modifications can be detected in precious tissue biopsies. Screen for processing-related impurities and host-cell related contaminants. A few months with Wes and we've already had way more success than we had in three years. page_head_start($title, $description); $status = strip_tags($HTTP_GET_VARS["status"]); Minneapolis, MN 55413 Department of Immunology, Landspitali, the National University Hospital Iceland, "Our lab came across ProteinSimples Simple Western platforms in 2014 and was surprised by the sensitivity and specificity of the technology, as well as its minimal usage of consumables. A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions. Which Brands are Currently Available on biotechne.com? I really like this system over traditional Western. 2023 ProteinSimple. With a processing time of 3 hours for 25 samples or 96 samples overnight, Simple Western dramatically speeds up time to results compared to traditional Western blotting protocols. Associate Professor, Department of Genetics and Genome Sciences, UConn Health, "Weve been able to increase productivity dramatically with Wes. Whether you are looking to process up to 24 samples per run or just 13, we have an instrument for you. Multiplex multiple targets in consecutive immunoassays with chemiluminescent detection. ", Nageswara Rao Boggavarapu, PhD, With Wes, we can do multiple assays with one sample collection. Director of Research Operations/Scientist, Roseman University of Health Sciences, "Wes gives me more throughput so I can get more replicates and trust my results a little bit more, and I can trust them straight away rather than having to do a lot of repeats. If your antibody of choice has not yet been screened in Simple Western, Novus Biologicals will reward you for being the first to testthe product in this application. Senior Postdoctoral Researcher. Create a publication or presentation lane view figure using annotation tools. if ($docid != 0) { Searching for key inflammation molecules", Kirstine Nolling Jensen, PhD Student, Setting the standard in quality research reagents for over 30 years, A trusted leader in quality life science reagents, Your trusted supplier for innovative and high performance life science reagents, Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis, Proprietary RNAscope technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity, A world leader in developing liquid biopsy based diagnostics, 614 McKinley Place NE Novus BiologicalsR&D Systems! Simple Western Systems Simple Western Antibody Database Simple Western Antibody Submission DEC1 VENDOR: Novus Biologicals CAT #: NB100-1800 This antibody is validated for Gel-Free, Blot Free, Hands Free Simple Western Systems. // End custom page code Do you need better reproducibility? "Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform. // He found that traditional Western blot uses up to 300 pieces of plastic and Wes uses two. ", Nageswara Rao Boggavarapu, PhD, What should I do if my Simple Western validated antibody did not work? $pass = strip_tags($HTTP_POST_VARS["pass"]); Professor and Chair, University of Birmingham, UK, "I was able to screen various antibodies in a single run within half a day. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease", John K. Blackwood PhD, Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows. Jess allows me to accurately assess drug compound potency, and with its high throughput ability I can screen multiple compounds quickly and efficiently. Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics, "The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. } page_head_start($title, $description); DisplayLogin(0, $path, $status, $message, $headfoot, $bannermenus, $userid, $page, $docid, $login); separation module or detection module. All systems operate at 20-60% relative, non-condensing humidity, a temperature range of 18-24 C, and a power range of 100-230 V. Transform your cancer & immuno-oncology research by utilizing next-generation Western blotting tools to give you richer views into your samples. antibodies for use on Milo, check out our tech note. We only had a small number of [iPSC-derived macrophages] because the cell line does not proliferate. Our Simple Western systems offer the right throughput for your needs. Abby simplifies the steps involved in the Western workflow. The performance of any antibody will need to be optimized for the specific sample type being studied. DisplayLogin(0, $path, $status, $message, $headfoot, $bannermenus, $userid, $page, $docid, $login); ProteinSimple branded instruments are available to quote. All Rights Reserved. The performance of any antibody selected will need to be optimized for the test system being examined. All Rights Reserved. If you don't see your antibody listed here, we can help! ", Jane Gray, Ph.D., Our Wes runs are completed in less than 4 hours, as opposed to 4 days with traditional methods. Senior Research Scientist, MiNA Therapeutics, London, UK, "Simple Western allowed me to screen quickly lots of samples with different antibodies while eliminating the manual washing and incubation steps. Meet Jamie Boisvenue from the Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry. Users are required to log in to Compass when the protein analysis software is launched, Data files are signed and encrypted to prevent unauthorized changes (, Each instrument maintains a history of user commands, Each assay and data file includes a history of signed changes to the file. Detection of Human cIAP 1/HIAP 2 by Western Blot. And it's got all the 21 CFR Part 11 compliance tools you need with the new Compass Authorization Server v3.0.0 (more details below). FAX 612 656 4400. In a series of live workshops, our field application scientists will share their tips, tricks, and best practices on Simple Western to improve your assays from start to finish. Assistant Professor, Department of Pharmacology and Toxicology, University of Texas Medical Branch, "We collect samples from laser microdissection and were using our entire sample on just one regular Western blot. global $HTTP_GET_VARS; All Rights Reserved.
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